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MRS agar
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MRS agar : ウィキペディア英語版
MRS agar
Often abbreviated to MRS, this type of bacterial growth medium is so-named by its inventors: de Man, Rogosa and Sharpe. Developed in 1960, this medium was designed to favour the luxuriant growth of ''Lactobacilli'' for lab study. It contains sodium acetate, which suppresses the growth of many competing bacteria (although some other Lactobacillales, like ''Leuconostoc'' and ''Pediococcus'', may grow). This medium has a clear brown colour.〔EMD Chemicals, (MRS Agar ), 2002.〕
==Typical composition==

MRS agar typically contains (w/v):
*1.0 % peptone
*0.8 % egg extract
*0.4 % yeast extract
*2.0 % glucose
*0.5 % sodium acetate trihydrate
*0.1 % polysorbate 80 (also known as Tween 80)
*0.2 % dipotassium hydrogen phosphate
*0.2 % triammonium citrate
*0.02 % magnesium sulfate heptahydrate
*0.005 % manganese sulfate tetrahydrate
*1.0 % agar
*pH adjusted to 6.2 at 25°C
The yeast and meat extracts and peptone provide sources of carbon, nitrogen and vitamins for general bacterial growth. The yeast extract also contains vitamins and amino acids specifically required by ''Lactobacilli''. Polysorbate 80 is a surfactant which assists in nutrient uptake by ''Lactobacilli''. Magnesium sulfate and manganese sulfate provide cations used in metabolism.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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